Mass spectrometry-based sequencing of lignin oligomers.
Identifieur interne : 003196 ( Main/Exploration ); précédent : 003195; suivant : 003197Mass spectrometry-based sequencing of lignin oligomers.
Auteurs : Kris Morreel [Belgique] ; Oana Dima ; Hoon Kim ; Fachuang Lu ; Claudiu Niculaes ; Ruben Vanholme ; Rebecca Dauwe ; Geert Goeminne ; Dirk Inzé ; Eric Messens ; John Ralph ; Wout BoerjanSource :
- Plant physiology [ 1532-2548 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Lignine, Paroi cellulaire, Populus, Xylème.
- méthodes : Analyse de séquence, Spectrométrie de masse.
- Structure moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Lignin.
- chemistry : Cell Wall, Populus, Xylem.
- methods : Mass Spectrometry, Sequence Analysis.
- Molecular Structure.
Abstract
Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula x Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.
DOI: 10.1104/pp.110.156489
PubMed: 20554692
PubMed Central: PMC2923877
Affiliations:
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wicri:level="1"><nlm:affiliation>Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent</wicri:regionArea><wicri:noRegion>B-9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Dima, Oana" sort="Dima, Oana" uniqKey="Dima O" first="Oana" last="Dima">Oana Dima</name></author><author><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name></author><author><name sortKey="Lu, Fachuang" sort="Lu, Fachuang" uniqKey="Lu F" first="Fachuang" last="Lu">Fachuang Lu</name></author><author><name sortKey="Niculaes, Claudiu" sort="Niculaes, Claudiu" uniqKey="Niculaes C" first="Claudiu" last="Niculaes">Claudiu Niculaes</name></author><author><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name></author><author><name sortKey="Dauwe, Rebecca" sort="Dauwe, Rebecca" uniqKey="Dauwe R" first="Rebecca" last="Dauwe">Rebecca Dauwe</name></author><author><name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name></author><author><name sortKey="Inze, Dirk" sort="Inze, Dirk" uniqKey="Inze D" first="Dirk" last="Inzé">Dirk Inzé</name></author><author><name sortKey="Messens, Eric" sort="Messens, Eric" uniqKey="Messens E" first="Eric" last="Messens">Eric Messens</name></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term><term>Lignin (chemistry)</term><term>Mass Spectrometry (methods)</term><term>Molecular Structure (MeSH)</term><term>Populus (chemistry)</term><term>Sequence Analysis (methods)</term><term>Xylem (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence (méthodes)</term><term>Lignine (composition chimique)</term><term>Paroi cellulaire (composition chimique)</term><term>Populus (composition chimique)</term><term>Spectrométrie de masse (méthodes)</term><term>Structure moléculaire (MeSH)</term><term>Xylème (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term><term>Paroi cellulaire</term><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mass Spectrometry</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Analyse de séquence</term><term>Spectrométrie de masse</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Structure</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Structure moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula x Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20554692</PMID><DateCompleted><Year>2010</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>153</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Mass spectrometry-based sequencing of lignin oligomers.</ArticleTitle><Pagination><MedlinePgn>1464-78</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.110.156489</ELocationID><Abstract><AbstractText>Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula x Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Morreel</LastName><ForeName>Kris</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dima</LastName><ForeName>Oana</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hoon</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Fachuang</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Niculaes</LastName><ForeName>Claudiu</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Vanholme</LastName><ForeName>Ruben</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Dauwe</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Goeminne</LastName><ForeName>Geert</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Inzé</LastName><ForeName>Dirk</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Messens</LastName><ForeName>Eric</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>Wout</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>06</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName><QualifierName UI="Q000379" 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Kim</name><name sortKey="Lu, Fachuang" sort="Lu, Fachuang" uniqKey="Lu F" first="Fachuang" last="Lu">Fachuang Lu</name><name sortKey="Messens, Eric" sort="Messens, Eric" uniqKey="Messens E" first="Eric" last="Messens">Eric Messens</name><name sortKey="Niculaes, Claudiu" sort="Niculaes, Claudiu" uniqKey="Niculaes C" first="Claudiu" last="Niculaes">Claudiu Niculaes</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name></noCountry><country name="Belgique"><noRegion><name sortKey="Morreel, Kris" sort="Morreel, Kris" uniqKey="Morreel K" first="Kris" last="Morreel">Kris Morreel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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